But, there are so far no reports of vinblastine and vincristine from above endophytic fungi. Isolation, purification and maintenance of the endophytic fungus producing vinblastine and vincristine. The relationship between certain grasses and endophytic fungi is a symbiosis and ranges from mutualistic to antagonistic. It contains alkaloids which inhibit the growth of fungi, bacteria, viruses, protozoans and also have anticancer properties. Endophytes constitute an important component of microbial diversity, and in the present investigation, seven plant species with rich ethnobotanical uses representing six families were analyzed for the presence of endophytic fungi from their natural habitats during monsoon mayjune and winter novemberdecember seasons of 2007. Isolation and characterization of bacterial endophytes of.
Many endophytes are in the phyla basidiomycota and ascomycota. The effectiveness of the surface sterilization was confirmed by making. A study was conducted for two years to isolate the fungal endophytes from different parts of solanum tuberosum l. Endophytic fungi from medicinal plants of virudhunagar district for antimicrobial activity 148 isolation of endophytic fungi leaves were thoroughly washed with mild detergent and running tap water and then airdried. Isolation and identification of endophytic fungi from various. The aim of this study was to examine whether the internal tissues of celandine are colonized by endo. Isolation and molecular identification of endophytic fungal strain study of fungal growth on various culture conditions to find out the. This group colonizes living, internal tissue of plants without causing any immediate, overt negative effects krings et al. Isolation of endophytic fungi from thermopsis lanceolata and their antioxidant activity 29 name of genus fungi number root stem leaf alternaria 5 3 2 aspergillus 2 2 0 chaetomium 1 0 0 fusarium 1 0 0 totalpercentage 956.
Alternariatenuissima, colletotrichum truncatum, and alternaria sp. Isolation, characterization, and bioactivity of endophytic. Four of them showed an activity in the antagonist test against escherichia coli, pseudomonas aeruginosa, vibrio cholerae and shigelladysentriae. Endophytic fungi from medicinal plant bauhinia forficata. Ilic j, cosic j, vrandecic k, dugalic k, pranjic a, martin j. Isolation of endophytic fungi from leaves of pasania edulis and their.
Isolation procedures for endophytic microorganisms. Endophytes are ubiquitous and have been found in all species of plants studied to date. The fungus colletotrichum falcatum was found to be the coregroup fungus with the colonization frequency of 12. Endophytic fungi from the chinese medicinal plant actinidia macrosperma were isolated and identified for the first time. Some of the isolates of endophytic fungi exhibited significant inhibitory activity on selected test organisms. Endophytic fungi the fungi discussed in this report were isolated by decontaminating the endophytic bacteria. Aim of this study is isolation of endophytic fungi strains from healthy tissues of thermopsis lanceolata and evaluation of antioxidant activity of fermented extracts of these isolated endophytic fungi strains. Five different taxa were represented by 11 isolates, and six isolates were grouped. Copyright mushroom research foundation 2016 diversity of. The fungal endophytes are a diverse group of organisms forming associations almost ubiquitously throughout the plant kingdom. Endophytic fungi are a promising source of novel biologically active compounds including antimicrobials. Effect of selected endophytic fungi and resistant tomato cultivars on meloidogyne spp.
Identification and bioactive potential of endophytic fungi. Endophytic fungal diversity in medicinal plants of western. Eight morphologically distinct endophytic fungi were selected from the medicinal plants elaeocarpus sphaericus coded as elspf 1 to 4 and myristica fragrans coded as myfrf 1 to 4. One group of fungal endophytes are the arbuscular mycorrhizal fungi. Endophytic fungi were isolated from the leaves of catharanthus roseus obtained from different areas of pune csirnational chemical laboratory and university of pune, india. Studies on endophytic fungi of calotropis procera l. Exploration of endophytic microorganisms from selected. Article info abstract article history total 85 endophytic fungi of 10 genera were isolated from 200 segments of fresh azadirachta indica leaves collected from panchmarhi biosphere reserve.
Isolation, purification and characterization of vinblastine. Fungal endophytes were isolated from healthy plant parts such as. A total of 72 strains of endophytic fungi were isolated and characterized morphologically as well as on the basis of its15. Antimicrobial activity of endophytic fungi isolated from. All endophytic fungi could be cultivated on artificial media and maintained as a pure culture. Antimicrobial activities of mycoleptodiscin b isolated. The endophytic fungi recovered belong to hyphomycetes 68. When grasses are infected with endophytic fungi, they can receive important benefits such as antiherbivory from insects and vertebrates. A novel method to scale up fungal endophyte isolations. The fungus colletotrichum falcatum was found to be the coregroup fungus with the colonization frequency of. Endophytic fungi from medicinal plants wiyakrutta et al. Ahlich k, sieber t 1996 the profusion of dark septate endophytic fungi in nonectomycorrhizal fine roots of forest trees and shrubs. Endophytic fungi may be from hypocreales and xylariales of the sordariomycetes pyrenomycetes class or from the class of loculoascomycetes. Isolation and antibacterial activity of endophytic fungi.
The effect of the 26 endophytic fungi on the growth of p. This study aims to identify endophytic fungi from medicinal plant mellochiaumbellataand investigate their antibacterial potencies. Isolation of endophytic fungi from agarwood, lai kim kee 36576 resource biotechnology programme department of molecular biology. The isolated endophytic fungal strains were identified based on their macro and microscopic morphology and by using standard monographs, following the description as reported by roy chowdhury et al. Pdf isolation and identification of endophytic fungi. An endophyte is an endosymbiont, often a bacterium or fungus, that lives within a plant for at least part of its life cycle without causing apparent disease. Pdf isolation endophytic fungi from leaves of azadirachta. The dissected tissues were placed on potato dextrose agar, with 50mgl chloramphenicol. Straindm06 associated with a medicinal plant ocimum sanctum.
Isolation and identification of endophytic fungi from. Plant use of endophytic fungi in defense occurs when endophytic fungi, which live symbiotically with the majority of plants by entering their cells, often fungus or bacteruim, are utilized as an indirect defense against herbivores. The fungi represented 27 genera of which two belonged to. Fungal endophytes were isolated from healthy plant parts.
Isolation, identification, and frequency of endophytic fungi. Endophytes usually inhabit aboveground plant tissues leaves, stems, bark, petioles and reproductive structures, which distinguishes. Studies had shown previously to protect watermelon citrullus lanatus and cucumber cucumis sativus seedlings from anthracnose disease elicited by wildtype c. Endophytic fungi are an important, yet relatively unstudied group of microbial plant symbionts. Therefore this study was initiated with the objective of examining the antimicrobial producing. Isolation and identification of endophytic fungi from 10. Endophytic fungi were isolated from various tissues root, stem, petiole, leaf, and fl ower stalk of 3 and 4yearold ginseng plants panax ginseng meyer cultivated in korea. Eversince, a number of endophytic fungi have been isolated from other taxus plants such as taxus chinensis 3, taxus. Among the 26 endophytic fungi, the inhibition of most strains varied from 6. Isolation and identification of endophytic fungi from 10 species palm trees. Endophytes represent a promising source of biologically active metabolites for pharmaceutical and agricultural applications. Endophytic fungus definition of endophytic fungus by. The aim of the present study was to identify the endophytic fungi form dicot and. The results show that none of the treatments equaled 100 mgl carbendazim, which was used as the positive control.
Fungi that live within functional and healthy plant tissues for all or part of their life cycle. Presence of endophytic fungi in monocot and dicot plant is in the most cases beneficial for the plant. Plant use of endophytic fungi in defense wikipedia. The aim of this study was to identify the endophytic fungi from medicinal plant ricinus communis and to investigate their potential antibacterial activity. A total of 25 fungal species were isolated from healthy leaves and identified based on the morphology of. The most frequently encountered endophytes are fungi staniek et al. Single isolate were transferred to fresh pda plates using the hyphal tip method. Most endophytes known are bacteria or fungi, although there are also some endophytic algae and oomycetes.
A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. The collection of leaves, stems, sepals, and seeds from healthy specimens of bauhinia forficata was made randomly in the didatic garden of the center of biological sciences, federal university of pernambuco, recife, brazil 83. Here, we describe a bulk surfacesterilisation device that can be used to surface. A total of six 6 endophytic fungi were isolated from 20 samples of a. Extracellular enzymatic activities of endophytic fungi. Twelve species of endophytic fungi weresuccessfully isolated from mellochiaumbellataleaves and twigs. The fungi used in this study are the endophytic fungi isolated from different medicinal plants, azadirachta indica, citrus limon, gossypium hirsutum, magnolia champaca, datura stramonium, piper betle, phyllanthus emblica collected from the in k.
To isolate and characterize an extracellular antifungal protein exafpc28 from endophytic fungi colletotrichum sp. Diversity of fungal endophytes in various tissues of panax. Endophytic fungi live asymptomatically, and sometimes systemically, within plant tissues carroll, 1988, 1991. Isolation and characterization of an extracellular. A number of endophytic fungi have been isolated by kharwar et al. They exhibited characteristic colony and microscopic morphology that could be used to differentiate them. The isolated endophytic fungi were identified based on the sequence analysis of the internal transcribed spacer its, 15. Pdf endophyte fungi reside in the tissues beneath the epidermal cell layers and live within a plant for at least part of its life without causing. Faculty of resource science and technology university malaysia sarawak. Aged i56ce222582010 a thesis submitted in partial fulfilment for the requirement of the.
All isolates were identified as belonging to 6 genera. After which they were surface sterilized by submerging them in 75% ethanol for 2 min. Endophytic fungi represent an important and quantifiable component of biodiversity. Results and discussion total of 14 endophytic fungi were isolated from 610 samples of both leaves and stem of different parts of endemic plants table 1. The colonization frequency cf % of each endophytic fungi was calculated and determined using the. Isolation endophytic fungi from leaves of azadirachta indica article pdf available in current science 7811. Endophytic fungi usually began to produce hypal filaments after 56 days of incubation at 30 0c. Resent report indicates that the endophytic fungi are responsible for the production of secondary metabolites 6 11. Endophytes are distinct from mycorrhizal fungi or rhizosphere microbes in that they live entirely within the plant. Isolation and characterization of an extracellular antifungal. Plant endophytic fungi of sri lanka, an island with exceptionally high biodiversity and endemism, are a vastly untapped resource. This study was conducted to characterize and explore the endophytic fungi of selected plants from the western himalayas for their bioactive potential. Endophytic fungi are fungi that live in plant tissues without causing disease symptoms and abnormalities in plants. Studies on biodiversity of fungal endophytes of indigenous.
Plant endophytic fungi resources is nearly a decade of research hot spots, endogenous fungal resources develop ment and utilization will be fruitful aim. In the present investigation ten medicinal plants were analyzed for the presence of endophytic fungi. Endophytic fungi are beneficial to their host plants and have the ability to produce bioac tive compounds that. Endophytic fungi are those living inside the host plant without causing any apparent negative effect on host plant. Isolation of endophytic fungi in the present study, fungal strains were isolated from leaves and twigs of mellochiaumbellata. Endophyte fungi reside in the tissues beneath the epidermal cell layers and live within a plant for at least part of its life without causing apparent diseases. As possible causes of such biases in withinleaf distributions of the endophytes. In exchange for carbohydrate energy resources, the fungus provides benefits to the plant which can include increased water or nutrient uptake and protection from. This study aims to obtain endophytic fungal isolates from local aromatic rice and screening of their ability to produce the iaa hormone as a plant growth promoter. Any fungi that are subsequently isolated from the plant samples are assumed to be endophytic, so it is crucial to have confidence in the surfacesterilisation process. Evaluation of antimicrobial activity of endophytic fungi. Dreyfuss and chapela 1994 estimated that endophytic fungi, alone could be. Gao 1school of plant protection, anhui agricultural university, hefei, china 2school of life science, anhui agricultural university, hefei, china. Pdf isolation and identification of fungal endophytes.
Transmission of class 1 endophytes is primarily vertical, with maternal plants passing fungi on to offspring via seed infections saikkonen et al. Sixtythree isolates of endophytic fungi were collected from root of maize plant var. A total of 81 thai medicinal plant species collected from forests were examined for the presence of endophytic fungi with biological activity. Isolation and antibacterial activity of endophytic fungi from.
Apr 07, 2018 the term endophytes includes a family of microorganisms that grow intraandor intercellularly in the tissues of higher plants without any symptoms on the plants in which they reside. This was the first study to evaluate their cytotoxic and antitumour activities against brine shrimp and five types of tumour cells, respectively. Four most active isolates from antagonist test which showed the biggest inhibition zone were fermented. Isolation procedures for endophytic microorganisms springerlink.
The fungus used in this study is one of the twenty endophytic fungi isolated from the leaves of medicinal plants in nashik city, india. Endophytic fungi are any fungi that grow all or part of their life cycles symptomlessly in. Pdf isolation and screening of endophytic fungi from three plants. Evaluation of antimicrobial activity of endophytic fungi from. Total 14 endophytic fungi were isolated from 9 medicinal plants. Diversity of endophytic fungi from the ornamental plant adenium obesum. Diversity of endophytic fungi from root of maize var. Alternaria alternata, cladosporium cladosporioides, chaetomium globosum, c. The term endophytes includes a family of microorganisms that grow intraandor intercellularly in the tissues of higher plants without any symptoms on the plants in which they reside. Abstract endophytic fungi isolated from four different populations of urginea indica are reported in the present report. The potency of endophytic fungal isolates collected from. Copyright mushroom research foundation 2016 diversity. The endophytic fungi were subcultured on pda medium.
Influence of endophytic fungi isolated from symptomless. Five different taxa were represented by 11 isolates, and six isolates were. Agarwood, or gaharu in malaysia, is one of the traditional woods that is highly demanded by the world market population. Pdf isolation and screening of endophytic fungi for the.
878 753 41 370 1652 527 361 163 89 714 726 512 239 37 22 1129 1197 1020 739 1581 30 225 1662 472 221 764 653 52 792 773 1173 297 315 735 374 1201 8 132 187 1108 1185 635 291 1128 624